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GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

The cell is protected from large shifts in redox equilibrium by active extracellular transport of GSSG or reaction with protein sulfhydryl groups and the formation of mixed disulfides

This process supports cellular health and promotes regeneration at the molecular level

An Annexin V-EnzoGold (enhanced Cyanine-3) conjugate enables the detection of externalized phosphatidylserine (PS) (a marker of early apoptosis) distinct from fluorescein or GFP signals

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For others, outcomes may mirror placebo effects or result primarily from behavioral changes accompanying treatment