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(4) Wash the column with approximately 10-15 bed volumes of Binding/Wash Buffer or until the absorbance is stable

Diarrhea is another commonly reported gastrointestinal side effect that may range from mild loose stools to more significant episodes that could lead to fluid and electrolyte imbalances if severe or prolonged

Silk fibroin solution preparation SF solution was prepared based on the literature 32

However, uncontrolled T cell immunity may cause autoimmune disorders

All procedures were carried out in strict accordance with the code of ethics