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In individuals with RA, the excessive production of oxygen free radicals results in damage to joint synovial cells and chondrocytes, intensifying the inflammatory response and pain [104]

10.1016/j.febslet.2012.09.036 84 MeuxE.ProsperP.NgadinA.DidierjeanC.MorelM.DumarcayS.et al

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Next day, cells were washed with PBS and incubated with Anti-mouse Alexa flour 488 (1:500 dilution) and Anti-rabbit Alexa flour 647 (1:500 dilution) secondary antibodies for 2 hrs at RT

Since this process does not require enzymatic mediation, membrane damage can rapidly propagate to adjacent healthy cells [12]

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