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The ID50, defined as the dilution at which relative light units were decreased by 50% compared to virus control wells (virus + cells) after background subtraction in cell-only control groups, was calculated using nonlinear regression in GraphPad Prism

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Trabi M, Schirra HJ, Craik DJ: Three-dimensional structure of RTD-1, a cyclic antimicrobial defensin from Rhesus macaque leukocytes

Biochem Biokhimiia 74:589598 Bonetti F, Brombo G, Zuliani G (2016) The relationship between hyperhomocysteinemia and neurodegeneration

This is where a conversation with a general practitioner or mental health specialist becomes essential, because untreated depression itself is linked to ED and changes in sexual behavior