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The cells were cultivated in Dulbeccos Modified Eagle Medium (DMEM) with high glucose, 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin, at 37 C in a humidified atmosphere containing 5% CO

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPS

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