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Most research suggests that observable changes, particularly in metabolic markers and body composition, may take several weeks to months

doi: 10.1161/ATVBAHA.107.143735 272 SantoroL.BreedveldG

in fact, food rich in antioxidants may play an essential role in the immune system, production of cellular energy, as well as scavenge the ROS

Afterwards cells were washed in RPMI 1640 (Life Technologies, without Glucose) and resuspended in RPMI 1640 (without Glucose, 100 M 2-NBDG (Life Technologies), 10% dialyzed FCS)