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What to measure: a practical menu A clinically useful redox panel should integrate four complementary layers into a single, interpretable picture: species-level reporters (mechanistic layer)use DHE-HPLC to quantify O 2 specifically via its diagnostic 2-OH-E product (Michalski et al

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The immunofluorescence data can be found at: Further enquiries can be directed to the corresponding author

There are different subcellular isoforms of each of the antioxidant enzymes and each isoform differentially responds to different stress and developmental cues

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The cells were then lysed with 2% digitonin in PBS and the protein concentration was determined using the BCA assay kit