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As shown in Figure 6, reporting the residual activity of LiTR after treatment with a fixed concentration (100 M) of the compounds, the fragments have a low but evident effect on protein activity, in line with the general behavior of fragments, whose affinity for the target is usually in the millimolar range due to the small size of these compounds that limits the number of possible interactions

The outcome of this competition depends from the Cu(II)-binding affinities and concentrations of HSA, CuHSA and His according to the following simplified reaction scheme: HSA and His are present in the blood serum at average 650 M and 75 M concentration respectively 22,42,43,44

Long-term consumption of food-derived chlorogenic acid protects mice against acetaminophen-induced hepatotoxicity via promoting PINK1-dependent mitophagy and inhibiting apoptosis