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To improve the expression, solubilization, and purification of recombinant proteins, it is therefore advised to alter the culture conditions by switching the medium or the temperature, co-expressing chaperones, adding large hydrophilic fusion tags as well as adding sucrose, raffinose, glycine, betaine and sorbitol during growth for enhancing the recombinant protein expression, solubilization and purification [5, 6]

in the steady state, the transepithelial passage of small amounts of food-derived antigens and microorganisms participates in the induction of a homeostatic immune response dominated by immune tolerance to dietary antigens 1, 2 and the local production of secretory immunoglobulin A (SIgA), 3 preventing pathogenic and commensal microbes from entering internal compartments

doi: 10.1016/j.actbio.2022.07.055 203 WangLTongLXiongZChenYZhangPGaoYet al

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The drug is injected into points on the face, back of the neck, back of the neck, and upper arm

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