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Many of these IEMs had not even been described when the diagnosis of Reyes syndrome was made

Comprehensive evaluation of the 5XFAD mouse model for preclinical testing applications: a MODEL-AD study

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Immunoblotting Following the indicated treatment, cell lysates were generated in ice cold RIPA lysis buffer (20 mM Tris-HCl (VWR, 97061-258), pH 7.5, 150 mM NaCl (Fisher Scientific, S271), 1 mM EGTA, 1 mM EDTA [Sigma-Aldrich, E5134], 1% sodium deoxycholate [Sigma-Aldrich, D6750], 1% NP-40 [Sigma-Aldrich, 74385]) supplemented with protease inhibitors (Fisher Scientific, PIA32955)

Interestingly, Boccaccio showed in a mouse model that activation of the oncogene induced cancer and a thrombohemorrhagicsyndrome through transcriptional upregulation of the procoagulation factors plasminogen activator inhibitor type 1 (PAI-1) and cyclooxygenase-2 (COX-2)