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GraphPad Prism 8 was used for statistical analysis

Structural comparisons with previously reported poliovirus structures (PV1 mature virion, PDB: 1HXS 29 , and PV1 expanded 135S particle, PDB: 6P9O 30 ) revealed that wtVLP closely resembles the PV1 135S-like particles (RMSD = 0.741 ), while the sVLP aligns well with the native PV1 structure (RMSD = 0.600 ) (Supplementary Fig