how to calculate total glutathione Rapid determination of reduced and oxidized levels using a new thiol-masking reagent and the enzymatic recycling method: Application the rat liver and bile samples Total GSH level (upper panel)

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The slices were maintained in a cell incubator at 37 C and 5% CO 2 for 14 days before use, and the culture medium was replaced every other day

A comprehensive panel of antibodies was utilized for Western blotting, targeting phosphorylated and total forms of ERK1/2, p38 MAPK, JNK1/2, and NF-B p65 (Ser536), with -actin serving as a loading control

10.1007/s11356-021-13411-w 83 ItaniR.SoubraL.KaroutS.RahmeD.KaroutL.KhojahH

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Rats who received GTP demonstrated less fibrosis, increased nerve conduction velocity and skeletal muscle wet weight, and increased number and size of myelinated fibers associated with higher expression of NGF, GAP-43 (axonal regeneration marker), Neurofilament (NF)200, and myelin-associated glycoprotein (MAG) in the regenerated nerve compared with vehicle-treated rats [37]