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Preparation of single cell suspension from tissue Foot skin Whole foot skin was cut into small pieces, which were digested for 45 to 60 minutes at 37C under gentle agitation in RPMI 1640 containing 0.2 mg/mL collagenase P and 0.1 mg/mL DNAse I (both Roche Diagnostics GmbH)

Rapamycin selectively expands CD4 + CD25 + Foxp3 + regulatory T cells

& Brooks, S.F

In females, transcriptional signatures indicated enhanced mitochondrial fission and ATP-generating capacity, consistent with reports that stress and cannabinoid exposures alter mitochondrial metabolism and bioenergetic coupling during adolescence [67]

Therefore, rapamycin could treat allergic dermatitis by inhibiting mTOR

J.KaufmanE.et al (2003)