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It is well established that exhaustive exercise increases circulating NH 3 (Reference Weiner and Verlander68) , and one possible explanation for the enhanced glutamine within the brain could be to detoxify the brain via the removal of NH 3 through the synthesis of glutamine using GS (Reference Conway and Hutson69)

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Glutathione reductase was added to reduce GSSG to GSH and total GSH was measured as described in the instruction manual