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Glutathione (GSH) is a tripeptide that contains an unusual peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain.It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides

Cherian A, Divya KP, Vijayaraghavan A

2 Department of Nutrition, Faculty of Public Health, Kerman University of Medical Science, Kerman, Iran

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The protein of interest can be cleaved off the GST tag by site-specific protease if desired

Hepatic lobules are primarily divided into three zones, with Zone 1 hepatocytes near portal vein (PV) areas and connecting points, Zone 3 hepatocytes around the central vein (CV), and Zone 2 hepatocytes positioned between them (Paris and Henderson, 2022)