Post-translational Regulation of ACO An in vitro phosphorylation assay using protein extracts of pre- and post-climacteric apple fruit and an ectopically expressed His-tagged Md ACO1 (MDP0000195885) hinted for the first time that ACO protein-protein interactions could exist (Matarasso et al., 2005), and the strongly conserved C28 as a key residue in the active site of this protease ( in silico predictions based on sequence similarity, other motifs for ACO protein-protein interactions, serine/threonine kinases, tyrosine kinases, and glycosylation were identified ( Arabidopsis ACO2 (AT1g62380) was identified as a target in a broad proteomics screen that characterized stress-induced protein S -glutathionylation, suggesting that ACO2 is post-translationally modified by glutathionylation ( Another thiol-residue modification of ACO was discovered in a large cysteine S-sulfhydration screen in Arabidopsis, which showed that ACO4 can get sulfhydrated ( 2 S production increases in stomata upon a prolonged ethylene treatment, and that this leads to the S-sulfhydration of C60 (equivalent to C63 of At ACO2) of Sl ACO1 (Solyc07g049530) and Sl ACO2 (Solyc12g005940)
Funding: This publication was made possible by grant # NPRP 4-571-3-171 from the Qatar National Research Fund (a member of Qatar Foundation)
10.3389/fmicb.2021.780768 Front Microbiol
doi:10.3109/13697137.2013.861814
Examples include: immune-system development disorders rare genetic conditions that affect lymphocyte survival or function Your doctor will consider these only if the pattern is unusual or begins early in life
Biotinylated surface proteins were enriched by incubating 100 g total protein lysates with 10 l streptavidin-agarose beads suspension (50% slurry, ThermoFisher Scientific) in 800 l total volume on a rotator at 4 C for 1 h
