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Local and Theoretical Concerns Local reactions observed or theoretically expected in animal models include: Transient erythema at topical application sites Mild irritation or inflammatory responses at injection sites Potential for altered MMP activity with excessive dosing Theoretical risk of excessive collagen degradation if matrix enzyme balance is disrupted The concern about copper toxicityvalid for free copper ionsappears mitigated by the peptides chelation mechanism

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After storage at 20 C overnight, diluted samples were thawed again, centrifuged for 10 min at 20,000 g and 4 C