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Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition

3-Hydroxydaidzein produces 3-hydroxypuerarin via PIUGT43, and daidzein produces formononetin via PIOMT9

The electron transfer generates the proton gradient necessary for ATP production

The second pattern of correlations linked exercise, gender and Internal locus of control with 5MTHF and seven phosphatidylglycerides

10.1016/j.matlet.2022.132079 46