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Briefly, The Cu(I)-catalyzed click reaction was initiated by adding Click-iT reaction buffer and 50 l of Azide-PEG3-biotin in 50 mM (Cayman Chemical, 23419) to each sample and incubated on a rotator for 20 min at room temperature

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From the moment you are born, your cells are hard at work, performing thousands of functions every second to keep you moving, thinking, and feeling your best