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For a common working concentration of 2.5 mg/mL, add 2 mL of solvent directly to the lyophilized vial and allow it to dissolve gently without vortexing, as aggressive agitation can cause peptide aggregation and loss of bioactivity

International Journal of Hygiene and Environmental Health, 218 (1), 1-11

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However, this is an off-label, unvalidated approach

10 of each serial dilution was then added to 90 L of blank plasma for final concentrations of 0.01 , 0.02, 0.05, 0.1, 0.2, 1 , 10, 20, 50 and 100 g/mL

FIGURE 1 3.2 FOXO4-DRI effectively improves vascular function in prematurely aging mice To further explore the role of FOXO4-DRI in aged mice, we constructed prematurely aging mouse model by administering D-galactose (200 mg/kg) intraperitoneally for 8 weeks