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doi: 10.1038/s42003-021-02825-4, 7

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To this end we investigated the consequences of T3 treatment in an experimental model of ischemic stroke in mice Methods: Stroke was induced in C57BL/6 WT mice by transient middle cerebral artery occlusion (tMCAO)

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This technique involves the introduction of genes that increase nutrient availability and decreases anti-nutrient compounds (Shahzad et al., 2021)

The amount of cysteine or cysteamine released was determined by adding 1 mM BrP forming cyclic sulfur products (lanthionine ketimine and aminoethylcysteine ketimine) that absorb at 296 nm ( = 6200 M 1 cm 1 ), while after adding NaOH (20 mM final concentration) to the solution the homocysteine released was determined by the reaction with 1 mM BrP