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We used 6 187 base-pair (bp) arrays for all array-based experiments except hydroxyl radical footprinting, which used 6 197 bp arrays, as the longer linker DNA improves resolution of the 10-bp periodicity in the linker stem region

We further compared these values with those reported in the literature

doi: 10.3390/biom13050730

Generally, they are higher in adult males than in adult females

After centrifugation at 500 g for 5 min, the supernatant was discarded, and pellets were resuspended in 0.5 mL of serum-free PBS for analysis by flow cytometry (CytoFLEX, Beckman Coulter, USA)

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