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Beyond these direct effects on redox status, Nrf2 also modulates the expression of genes implicated in cellular defense, thereby inhibiting redox cycle reactions and diminishing GSH consumption (52)

In the second step (RT-2), a 13 L master mix was prepared containing 5 L of 5 M-MLV RT buffer, 2 L of DTT (10 mM), 0.25 L of dNTP mix (40 mM, Promega), 0.25 L of RNase inhibitor (40 U/L, Promega), and 0.5 L of M-MLV Reverse Transcriptase (200 U/L, Promega), with the remaining volume adjusted using nuclease-free water

Samuni et al., 2013)

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Rather than boosting hormones, the goal of supplementation in midlife is to support the pathways estrogen normally regulates, helping the body adapt more smoothly to hormonal change