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Imaging glutathione depletion in the rat brain using ascorbate-derived hyperpolarized MR and PET probes

This manuscript reflects only the authors view and IMI JU is not responsible for any use that may be made of the information it contains

[DOI] [PMC free article] [PubMed] [Google Scholar] 279.Klein Klouwenberg P.M.C., Frencken J.F., Kuipers S., Ong D.S.Y., Peelen L.M., van Vught L.A., Schultz M.J., van der Poll T., Bonten M.J., Cremer O.L., et al

PubMed Central, Moretti, Rita, and Paola Caruso

4060 g of protein per sample was electrophoresed and transferred onto nitrocellulose membrane (Bio-Rad) for immunoblotting using antibodies against IRE1 (3294, Cell Signaling), XBP1 (ab220783, Abcam), PERK (3192, Cell Signaling), phospho-GCN2 (ab75836, Abcam), GCN2 (3302, Cell Signaling), phospho-eIF2 (9721, Cell Signaling), eIF2 (2103, Cell Signaling), ATF4 (11815, Cell Signaling), ATF6 (CosmoBio, BAM-73-500-EX), GCLC (sc-390811, Santa Cruz Biotechnology), GCLM (sc-22754, Santa Cruz Biotechnology), GPX4 (MAB5457, R&D Systems), SLC7A11 (12691, Cell Signaling), ACSL4 (SAB2701949, Sigma Aldrich), phosphor-JNK (9251, Cell Signaling), JNK (9252, Cell Signaling), LC3B (NB600-1384, Novus), Caspase-3 (9662, Cell Signaling), luciferase (NB600-307, Novus Biologicals), calreticulin (ab2907, Abcam), HSP90 (sc-13119, Santa Cruz Biotechnology), GAPDH (8884, Cell Signaling) and -actin (sc-1615, Santa Cruz Biotechnology) all with 1:1000 dilution in 3% (w/v) BSA (Sigma Aldrich)

Unique Advantages of AOD-9604: The key distinguishing feature of AOD-9604 is its specific research evidence for cartilage regeneration in osteoarthritis models, combined with an excellent safety profile from extensive human clinical trials