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Physiol Res 65(4):547560 Talbert EE, Smuder AJ, Min K, Kwon OS, Powers SK (2013) Calpain and caspase-3 play required roles in immobilization-induced limb muscle atrophy

CRM1 was released by on-column cleavage with TEV protease

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Common TB-500 Mistakes Shaking the vial Don't shake the vial

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Binding induces autophosphorylation of the intracellular kinase domain on tyrosines 1131/1135/1136, recruits substrates IRS-1, IRS-2 and Shc, and activates two major signaling pathways: the PI3K-AKT-mTOR pathway (protein anabolism, cell growth, apoptosis inhibition) and the Ras-Raf-MEK-ERK pathway (cell proliferation, growth gene transcription)