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Design of reversible, cysteine-targeted Michael acceptors guided by kinetic and computational analysis

Gene expression was determined by qPCR and protein expression was determined by Western blot and immunofluorescence and proliferation of cells by Xcelligence and BrdU assay

Because many people have insufficient levels of glutathione, including those who are not ill

Journal of Hepatology 50 , 11841191, doi: 10.1016/j.jhep.2009.01.030 (2009)

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Notably, glutathione balance and GPX4 levels are deeply involved in the susceptibility of cardiomyocytes to ferroptosis, a novel form of cell death