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Then, amplification was carried out by 45 cycles of 94 C for 10 s, annealing temperature for 30 s, and 72 C for 20 s

8 DISCLOSURES The authors report no relevant financial relationships which, in the context of their contributions, could be perceived as a potential conflict of interest

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In addition, when glutamine is limiting via glutaminase inhibitor, asparagine can modulate glutamine synthesis by up-regulating glutamine synthetase, thereby participate in the regulation of the TCA cycle

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