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References: Sugimoto K, Murakawa Y, Sima A

1d, e), justifying the use of 5 sgRNAs per gene in the genomewide library

MS/MS spectra were searched using MaxQuant version 2.0.3.1 software 70 against uniprot_taxonomy_9606_unique.fasta database

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The hydroxyl groups of ribose make hydrogen bonds with the side chain of Asp161 and several main chain atoms from the bottom of the active-site cavity (including Gly158, Phe156, and Pro38)

The results found that Mettl3 showing the largest difference in the ALI group compared to the control group (Figure 3C)