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Afanas'ev, I

Primary patient-derived AML cells were isolated using Ficoll gradients and were cultured in IMDM (Thermo Fisher Scientific, USA) supplemented with 15% BIT 9500 Serum Substitute (STEMCELL Technologies, Canada), 100 ng/ml stem cell factor (SCF) (ImmunoTools, Germany), 50 ng/ml FLT3L (ImmunoTools, Germany), 20 ng/ml interleukin-3 (IL-3) (ImmunoTools, Germany), 20 ng/ml G-CSF (ImmunoTools, Germany), 0.1 mM 2-mercaptoethanol (Gibco, USA), 50 g/ml gentamicin (Thermo Fisher Scientific, USA), 10 g/ml ciproflaxin (Thermo Fisher Scientific, USA), 500 nM SR1 (APExBIO, USA) and 1 M UM279 (APExBIO, USA) at 37 C, 5% CO 2 , and 95% humidity

This supplement provides the complete, pre-formed glutathione molecule directly for more immediate support

Therefore, it is considered that the dosing strategy can be further optimized (Table 1)

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