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The next day, cells were treated with chemicals (erastin, Fer-1, and LY2090314) for the indicated times, incubated with 5 M of Calcein-AM for 20 min, then washed with PBS for three times

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10.1016/j.neuro.2006.10.006 Neurotoxicology 174 Roldan-TapiaL.ParronT.Sanchez-SantedF

The membranes were blocked with 5% non-fat milk and incubated with the following antibodies at indicated dilutions: anti-ID1 (1:1,000

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To normalize the pulse and keep it in the optimal/safe zone in case of physical overstrain