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To further analyze the effect of 3-MA on vitiligo fibroblast growth, parallel immunofluorescence analysis employing the proliferation marker Ki67 demonstrated a decrease in percentage of cycling fibroblasts after 3-MA, which appeared significant at 48 h of treatment

Tubular structures are defined as those with a length four times their width

The resin was washed with 40 times volume of dissolving buffer and 40 times volume of washing buffer (10 mM Tris, 100 mM NaH 2 PO 4 , pH 8.0, 8 M urea, and 20 mM imidazole)

Using the second harmonic generation properties of fibrilar collagen and multi-photon microscopy, we confirmed that fibroblasts from former smokers with COPD were unable to efficiently remodel collagen into fibrils (Figure 6d)

doi: 10.3390/nu11061356