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5.11 Vitamin B13 In mammals, orotate (OA) or vitamin B13 is released from the mitochondrial dihydroorotate dehydrogenase (DHODH) and converted to UMP by the cytoplasmic UMP synthase enzyme

The images were further processed and merged in ImageJ (v1.53e)

3.4.4 Trimethylamine Gut microbiota metabolism involves the conversion of choline into TMA, which is absorbed by the host and subsequently converted into trimethylamine N-oxide (TMAO) in the liver (Arias et al., 2020)

Both vitamins have excellent safety profiles, with B12 being water-soluble and excess amounts easily excreted

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Western blot analysis The tissue homogenates were processed for immunoblotting studies