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Cell Death Differ (2018) 25(2):22940

15(6): e098903

Another crucial point is that, when an individuals antioxidant defense system is weak or saturated, inhaling additional ROS or other reactive metabolites produced by the biotransformation of chemicals in tobacco smoke can increase the amount of oxidative stress caused by the gas-phase and tar-phase derived ROS (60)

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Moreover, the same NO/cGMP signaling was effective in survival cell response to GSH depletion in skeletal muscle cells ( On the basis of these knowledge, it is not surprising that alterations in GSH homeostasis have been implicated in the etiology and/or progression of many human diseases ( In this review we briefly summarize the mechanisms of GSH synthesis and homeostasis, while we will focus on the strict connection between GSH levels and oxidative/nitrosative stress and on the downstream redox changes that modulate signaling pathways involved in viral infection and autophagy (ATG)

For F-actin quantification, at the end of experiment, cells were trypsinized then fixed with PBS/ formalin 3% and incubated with PBS/ Triton 0.1%/ TRITC-phallodin 1 g/mL (Sigma Aldrich) during 5 min