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For LCMS analysis, metabolite extracts were analysed by high-resolution mass spectrometry with an Orbitrap Exploris 240 (Thermo) coupled to a Vanquish Flex liquid chromatography system (Thermo)

1 ER-IP approach enables rapid purification of ER from mammalian cells

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To execute this, 200 L of the examined samples, previously diluted with PBS at a 1:5 ratio (v/v), were combined with standard solutions ranging from 0 to 100 mol/L alongside 200 L of blank PBS solution

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Probiotics ingestion does not directly affect thyroid hormonal parameters in hypothyroid patients on levothyroxine treatment