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Then the ultrapure GST-tagged recombinant proteins are eluted by excess reduced Glutathione

Nature 351, 153155

Accordingly, serine deprivation or SSP blockade restores the sensitivity of colorectal cancer cells to 5-FU ( 6.2.2 Targeted therapy resistance 6.2.2.1 BRAF inhibitor resistance Proteomic analysis of acquired-resistance cell lines revealed altered expression of serine biosynthetic enzymes (PHGDH, PSPH, PSAT1) in drug-resistant cells