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After washing with demineralized water, the sections underwent an antigen retrieval step in microwave-warmed (300 W) 0.1 M Tris/hydrochloride (HCl) buffer (pH 9.0), for 15 min, followed by a cooling-down period at room temperature (RT)

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Vulnerable urban populations, including homeless and those residing in low-income settings, are exposed to a potent mix of biological insults due to factors like poor ventilation in shelters, household crowding, and limited access to healthcare, all of which facilitate disease transmission

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