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Belkacem et al

By binding with its steroidal ligands, dafachronic acids (DAs), DAF-12 induces reproductive development under favorable conditions such as an abundant food supply, whereas DAF-12 together with co-repressor DIN-1S promotes dauer diapause under harsh environmental conditions such as limited food and overcrowding 6,7

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According to the 2023 American Diabetes Association guidelines, 150 min or more of moderate-intensity physical activity per week, similar in intensity to brisk walking, is recommended for preventing diabetes [75]

Statistics and reproducibility All experiments were repeated at least twice with independently prepared samples

Microfluidic organ-on-chip system for multi-analyte monitoring of metabolites in 3D cell cultures