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Each pixel was transformed into a phasor plot according to the following equations: in which g i , j ( ) and s i , j ( ) are the x and y coordinates of the phasor plot, n and are the harmonic frequency and the angular frequency of excitation, respectively, and T is the repeat frequency of the acquisition

The data were tested for a normal distribution (KolmogorovSmirnov and ShapiroWilk tests) and homogeneity of variance (Levene test)

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