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Immediately following addition of the substrate, fluorescence was monitored using a M5 Spectramax plate reader using SoftMax Pro 5 at 37 C, excitation at 529 nm and emission at 600 nm, with a 590 nm cut-off filter

[DOI] [PMC free article] [PubMed] [Google Scholar] 63.Bellido F., Pineda M., Aiza G., Valds-Mas R., Navarro M., Puente D.A., Pons T., Gonzlez S., Iglesias S., Darder E., et al