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The target zone is a band running roughly two inches on either side of the navel, staying at least two inches away from the navel itself

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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

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