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Isolated naive CD8 + T cells were resuspended at a concentration of 1 10 6 cells per ml in RPMI 1640 medium (Gibco) supplemented with 10% fetal calf serum, 100 U ml 1 penicillin, 100 g ml 1 streptomycin, 50 M 2-mercaptoethanol, 1 mM sodium pyruvate (Gibco), 1 non-essential amino acids (Gibco), and 100 U ml 1 recombinant human IL-2 (Imunace