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1 Phosphate buffer solution (PBS) and deionized water were used in the experiments

Administration typically follows a protocol of once daily injections for five days per week followed by two days off , rather than timing within a specific window.[2] This cycling approach has become the gold standard among researchers and practitioners

However, the limited stability and mechanical properties of HA restrict its direct application, leading researchers to commonly employ HA-based modified hydrogels for treating intervertebral disc degeneration

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Regular ghee may be made from A1 cow milk

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