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Intracellular Ca 2+ imaging Cells were transfected using Nucleofector as described before using the plasmids pCMV_R-GECO1 (Addgene #32444, measures intra-cytosolic Ca 2+ , red fluorescence) and pCMV_CEPIA2mt (Addgene #58218, measures intra-mitochondrial Ca 2+ , green fluorescence)

Pain in the brain, you cannot ignore it, right

Miller WP, Rothman SM, Nascene D, Kivisto T, DeFor TE, Ziegler RS, Eisengart J, Leiser K, Raymond G, Lund TC, Tolar J, Orchard PJ (2011) Outcomes after allogeneic hematopoietic cell transplantation for childhood cerebral adrenoleukodystrophy: the largest single-institution cohort report

Bears began to be protected in Slovenia in 1935 and the wolf gained full protection in 1993

Sokoloff and Le Foll, 2017)

Oral butyrate reduces oxidative stress in atherosclerotic lesion sites by a mechanism involving NADPH oxidase down-regulation in endothelial cells