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Pull-down assay Five micrograms of the bait protein c-MET927-TSI3-His was immobilized to TSE resin by incubating for 30 min on ice and washed with binding buffer PBS (Gibco) supplemented with10 M Tinzaparin and 2 mM CaCl2

Because the pancreas provides critical enzymes, as well as calcium required for vitamin B 12 absorption, pancreatic insufficiency may contribute to vitamin B 12 deficiency

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Proper absorption of vitamin B12 requires the normal function of the digestive system, specifically the stomach, pancreas, and small intestine