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Liver glutathione content Liver tissue was homogenized in cold buffer (50 mM potassium phosphate and 1 mM EDTA, pH 7.5) in ratio (1:5 w/v) using tissue homogenizer (Polytron homogenizer, PT 3100, Switzerland), then the homogenate was centrifuged at 3000 rpm for 15 min at 4 C (Sigma centrifuge 3 K15 SIGMA Laborzentrifugen GmbH, Germany), and the supernatant was collected

CrossRef Noninvasive imaging biomarkers for liver fibrosis in nonalcoholic fatty liver disease: current and future Jung Hwan Yu, Han Ah Lee, Seung Up Kim Clinical and Molecular Hepatology .2023

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We found that BiP gene expression ( Hspa5 ) was increased in G6pdh chon growth plates, which was accompanied by an increase in eIF2 phosphorylation, ATF4 protein expression and CHOP ( Ddit3 ) gene expression, indicative of the activation of protein kinase-like ER kinase (PERK)-mediated UPR (Fig