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2018 Jan 3;97(1):1-2

Flies were then washed (twice for 1 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and were reacted with 2.5 mM Ce-DAB2 solution in cacodylate buffer 36 for 75 min to produce the desired brown intensity color from the precipitate

The asarone in VOA is found to be principally responsible for the toxic effects of VOA

Therefore, once again, a combination of the two ligands may be thought to bind with more affinity to the enzyme

However, the number of ongoing studies indicates the therapeutic potential of the KD, and implementation of this results of these trials will enable the verification of the hypothesis