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In all experiments, signal quality was monitored by the Monolith device to detect possible ligand auto-fluorescence, aggregation, or target protein adsorption to capillaries

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The mRNA and protein expression levels of the 5-HT1A receptor in the hypothalamus of menopausal depression rats by CUMS model were significantly lower than those of normal rats, while the mRNA and protein expression levels of the 5-HT2A receptor were significantly higher than those of normal rats

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This finding suggests that, further to the toxicity of ammonia, there are other determining factors in triggering hepatic encephalopathy, such as inflammation, which has been proposed as an important factor (Shawcross et al., 2011)