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A higher extracellular concentration combined with a lower gradient is responsible for a higher intracellular concentration of H 2 O 2 at this toxic level

Immunofluorescence IC-21 cells infected with MCMV (K181) were collected at 4 dpi and the cell suspension was pre-incubated on ice for 30 min with 10% normal goat serum (NGS) plus 2% FCS in PBS to block nonspecific reactivity

Peroxide treatment A population of synchronized L1 larvae was added to 30-ml glass tubes containing 2.5 ml of S-complete medium (liquid culture) 76 and NA22 or induced RNAi bacteria at an OD 600 of 1.9

Samples were analyzed by GC/MS on an Agilent GC/MS 7890A/5975A with a DB-5 column

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In the intestinal host cells, indoleamine and tryptophan 2,3-dioxygenases (IDO/TDO) activate the kynurenine (KYN) pathway, generating KYN metabolites, many of which activate aryl hydrocarbon receptor (AhR) signaling