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Together, these findings indicate that reactive nitrogen species promote TTR crosslinking through reversible thiol activation, whereas strong oxidants divert cysteine chemistry toward stable overoxidized states that suppress disulfide-mediated conversion

Glutathione - Synthesis SFRBM Education Program Dickinson et al.7 de novo synthesis - 1 Enzymatic synthesis occurs from the component amino acids (glutamate, cysteine, and glycine) via the sequential action of two ATP-dependent, cytosolic enzymes