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Effects of amino acids from selenium-rich silkworm pupas on human hepatoma cells

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Jhunjhunwala S, Hammer C, Delamarre L

Cells were washed with PBS to remove excessive NEM before lysing in triton lysis buffer (50 mM TrisHCl, pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 150 mM NaCl) supplemented with protease inhibitor cocktail (EMD Millipore, 539134)

10.1007/s00604-018-2953-9 260 YangH.LiuY.GuoZ.LeiB.ZhuangJ.ZhangX.et al (2019)